A simple, precise, accurate and reliable HPTLC method has been developed and validated for analysis of Eperisone hydrochloride (EPE) and in their combined dosage form. Identification and analysis were performed on 100 mm x 100 mm layer thickness 0.2 mm, pre-coated silica gel G60-F254 aluminum sheet, prewashed with methanol and dried in an oven at 50°C for 5 min. Toluene : methanol : ethyl acetate : ammonia (6:2:2:0.05) (v/v/v/v) was used as mobile phase. Calibration plots were established showing the dependence of response (peak area) on the amount chromatographed. The validated calibration ranges were 450-1950 ng/spot for EPE with correlation coefficient (R2) 0.9958. Average % recovery was between 99.55–100.11% for EPE. The spots were scanned at 255 nm in a reflectance mode. The proposed method has been validated as per ICH guidelines and successfully applied to the estimation of EPE in their tablet dosage form.
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